文章信息
文章題目：Targeted MYC2 stabilization confers citrus Huanglongbing resistance
期刊：Science
發(fā)表時(shí)間：2025年4月11日
主要內(nèi)容：中國(guó)科學(xué)院微生物研究所葉健團(tuán)隊(duì)在Science期刊上發(fā)表了題為“Targeted MYC2 stabilization confers citrus Huanglongbing resistance”的研究論文。該研究成功解析柑橘抗黃龍病核心分子機(jī)制，并利用人工智能（AI）技術(shù)篩選出可有效防控該病害的小肽。這項(xiàng)研究不僅破解了困擾國(guó)際農(nóng)業(yè)界缺乏柑橘黃龍病抗性基因的科學(xué)難題，也為全球柑橘產(chǎn)業(yè)可持續(xù)發(fā)展提供了新的解決方案。
原文鏈接：https://science.org/doi/10.1126/science.adq7203
使用TransGen產(chǎn)品：
TransScript? One-Step gDNA Removal and cDNA Synthesis SuperMix (AT311）
ProteinFind? Anti-His Mouse Monoclonal Antibody (HT501）
ProteinFind? Anti-GST Mouse Monoclonal Antibody (HT601）
研究背景
黃龍病是全球柑橘產(chǎn)業(yè)面臨的毀滅性病害，因其潛伏期長(zhǎng)、癥狀隱匿、傳播迅速成為制約我國(guó)乃至世界柑橘產(chǎn)業(yè)高質(zhì)量發(fā)展的重大問(wèn)題。長(zhǎng)期以來(lái)，由于病原菌無(wú)法分離培養(yǎng)，且自然抗性種質(zhì)資源極為稀缺，導(dǎo)致相關(guān)研究和防控技術(shù)開(kāi)發(fā)進(jìn)展緩慢。
文章概述
本研究首次明確了E3泛素連接酶PUB21通過(guò)降解關(guān)鍵抗病蛋白MYC2，負(fù)向調(diào)控植物免疫響應(yīng)的作用路徑，發(fā)現(xiàn)了一個(gè)高度保守且可調(diào)控的遺傳抗性網(wǎng)絡(luò)。其中，鑒定的感病基因PUB21為柑橘抗黃龍病育種提供了明確的分子靶標(biāo)，對(duì)柑橘抗性改良具有重要實(shí)踐意義?；谶@一機(jī)制發(fā)現(xiàn)，研究團(tuán)隊(duì)自主開(kāi)發(fā)了靶向PUB21活性的高通量篩選平臺(tái)，成功篩選出雙功能肽APP3-14等具有顯著抑制黃龍病菌活性的候選分子，為黃龍病的防控提供了小分子物質(zhì)。該成果不僅豐富了植物免疫調(diào)控理論體系，更為我國(guó)柑橘黃龍病的綠色防控提供了理論支撐。
全式金生物產(chǎn)品支撐
優(yōu)質(zhì)的試劑是科學(xué)研究的利器。全式金生物的一步法去除gDNA及第一鏈cDNA合成試劑(AT311)、抗His標(biāo)簽鼠單克隆抗體 (HT501) 和抗GST標(biāo)簽鼠單克隆抗體 (HT601) 助力本研究。產(chǎn)品自上市以來(lái)，深受客戶青睞，多次榮登知名期刊，助力科學(xué)研究。
TransScript? One-Step gDNA Removal and cDNA Synthesis SuperMix (AT311）
本產(chǎn)品以RNA為模板，在同一反應(yīng)體系中，合成第一鏈cDNA的同時(shí)去除RNA模板中殘留的基因組DNA。反應(yīng)結(jié)束后，只需在85℃加熱5秒鐘，即可同時(shí)失活TranScript? RT/RI與gDNA Remover。
產(chǎn)品特點(diǎn)
? 在同一反應(yīng)體系中，同時(shí)完成反轉(zhuǎn)錄與基因組DNA的去除，操作簡(jiǎn)便，降低污染機(jī)率。
? 產(chǎn)物用于qPCR：反轉(zhuǎn)錄15分鐘；產(chǎn)物用于PCR：反轉(zhuǎn)錄30分鐘。
? 反應(yīng)結(jié)束后，同時(shí)熱失活RT/RI與gDNA Remover。
? 合成片段≤12 kb。
ProteinFind? Anti-His Mouse Monoclonal Antibody (HT501)
抗His標(biāo)簽鼠單克隆抗體為高純度的小鼠單克隆抗體，屬IgG1同型，免疫原為人工合成的6×His標(biāo)簽多肽序列(HHHHHH)。
產(chǎn)品特點(diǎn)
? 高純度的抗小鼠單克隆抗體，特異性強(qiáng)。
? 高度特異識(shí)別重組蛋白C末端或N末端的6×His標(biāo)簽。
? 適用于定性或定量檢測(cè)His融合表達(dá)蛋白。
ProteinFind? Anti-GST Mouse Monoclonal Antibody (HT601）
抗GST標(biāo)簽鼠單克隆抗體為高純度的小鼠單克隆抗體，屬IgG2a同型，免疫原為酵母Y258 GST重組表達(dá)蛋白。
產(chǎn)品特點(diǎn)
? 高純度的小鼠單克隆抗體, 特異性強(qiáng)。
? 與GST結(jié)構(gòu)域具有高親和性，適用于特異性檢測(cè)GST融合表達(dá)蛋白。
全式金生物的產(chǎn)品再度亮相Science期刊，不僅是對(duì)全式金生物產(chǎn)品卓越品質(zhì)與雄厚實(shí)力的有力見(jiàn)證，更是生動(dòng)展現(xiàn)了全式金生物長(zhǎng)期秉持的“品質(zhì)高于一切，精品服務(wù)客戶”核心理念。一直以來(lái)，全式金生物憑借對(duì)品質(zhì)的執(zhí)著追求和對(duì)創(chuàng)新的不懈探索，其產(chǎn)品已成為眾多科研工作者信賴的得力助手。展望未來(lái)，我們將持續(xù)推出更多優(yōu)質(zhì)產(chǎn)品，期望攜手更多科研領(lǐng)域的杰出人才，共同攀登科學(xué)高峰，書寫科研創(chuàng)新的輝煌篇章。
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使用ProteinFind? Anti-His Mouse Monoclonal Antibody（HT501）產(chǎn)品發(fā)表的部分文章：
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? Li Y, Shen H, Zhang R, et al. Immunoglobulin M perception by FcμR[J]. Nature, 2023.(IF 50.5)
? Lan Z, Song Z, Wang Z, et al. Antagonistic RALF peptides control an intergeneric hybridization barrier on Brassicaceae stigmas[J]. Cell, 2023.(IF 45.5)
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? Zhong S, Li L, Wang Z, et al. RALF peptide signaling controls the polytubey block in Arabidopsis[J]. Science, 2022.(IF 44.7)
使用ProteinFind? Anti-GST Mouse Monoclonal Antibody（HT601）產(chǎn)品發(fā)表的部分文章：
? Zhao P Z, Yang H, Sun J Y, et al. Targeted MYC2 stabilization confers citrus Huanglongbing resistance [J]. Science, 2025.(IF 44.7)
? Lu P, Zhang G H, Li J, et al. A wheat tandem kinase and NLR pair confers resistance to multiple fungal pathogens[J]. Science, 2025.(IF 44.7)
? Feng L, Luo X, Huang L, et al. A viral protein activates the MAPK pathway to promote viral infection by downregulating callose deposition in plants[J]. Nature Communications, 2024,.(IF 14.7)
? Li J, Liu X, Chang S, et al. The potassium transporter TaNHX2 interacts with TaGAD1 to promote drought tolerance via modulating stomatal aperture in wheat[J]. Science Advances, 2024.(IF 11.7)
? Fu X, Zhang J, Sun K, et al. Poly (ADP-ribose) polymerase 1 promotes HuR/ELAVL1 cytoplasmic localization and inflammatory gene expression by regulating p38 MAPK activity[J]. Cellular and Molecular Life Sciences, 2024.(IF 6.2)
? Zhou B, Luo Q, Shen Y, et al. Coordinated regulation of vegetative phase change by brassinosteroids and the age pathway in Arabidopsis[J]. Nature Communications, 2023.(IF 14.7)
? Gu P, Tao W, Tao J, et al. The D14‐SDEL1‐SPX4 cascade integrates the strigolactone and phosphate signalling networks in rice[J]. New Phytologist, 2023.(IF 8.3)
? Li N, Duan Y, Ye Q, et al. The Arabidopsis eIF4E1 regulates NRT1. 1‐mediated nitrate signaling at both translational and transcriptional levels[J]. New Phytologist, 2023. (IF 8.3)
? Liu Y, Yu T F, Li Y T, et al. Mitogen‐activated protein kinase TaMPK3 suppresses ABA response by destabilising TaPYL4 receptor in wheat[J]. New Phytologist, 2022.(IF 8.3)
